About Instrument and its applications-

Nuclear Magnetic Resonance (NMR) is a non-destructive analytical technique that studies the structure, composition, and behavior of molecules.
The machine is capable of recording 1H, 13C, 15N, 31P etc. NMR spectra which are routinely performed to get structural information of small molecules as well as polymeric compounds. 2D and 3D NMR spectra are also recorded for studying protein structure determination and DNA drug interaction studies.

Applications-
• Molecular structure: NMR can determine the molecular structure of a sample.
• Composition: NMR can monitor the composition of mixtures and quantify known and unknown components.
• Kinetics: NMR can be used to follow reaction kinetics to investigate reaction mechanisms.
• Dynamics: The most powerful technique for quantifying molecular dynamics and interactions of biomacromolecules.
• Purity: NMR can determine the purity of a sample.
• Physical properties: NMR can study physical properties at the molecular level.
• Drug screening and design: Particularly useful for identifying drug leads and determining the conformations of the compounds bound to enzymes, receptors, and other proteins.

General Information-
Make- Bruker
Model- AVANCE NEO Ascend 700 MHz Spectrophotometer
Year of Installation- 2025

Specifications-
1) Four independent RF-channels console with Z-gradient amplifier
2) 5 mm nitrogen cryogenic Prodigy BBO probe with BB range 31P-15N
3) 5 mm proton-optimized triple resonance (CH/l3C/15N) NMR probe with room temperature RF coils with 2H locking, Z-shielded gradient and Auto-tuning capability
4) Simultaneous decoupling of l3C, 15N and 2H nuclei
5) Sample Case autosampler for up to 24 samples
6) BCU II cooling unit cools probes down to -80°C - 150°C
7) Software: Topspin 4.4.1
8) Probes: BBO, TXI

Sample Requirements-
1) Powder: 15-30 mg (Powder should be finely ground and homogeneous)
2) Liquid: 500 – 700 µl

Note: If submitting a powder sample, it must be soluble in a suitable deuterated solvent to prepare a homogeneous solution.

Contact Person-
Dr. Jay Singh Meena
Technical Officer-II
Tel: +91-1332-285779/286170
E-mail: jsmeena.mic2018@iitr.ac.in